No, you can use any buffer and any additive as long as it does not negatively affect the stability or homogeneity of your sample.
Yes, for example an aggregation of your protein is easily detected by the MST instruments.
No, you can use any buffer and any additive as long as it does not negatively affect the stability or homogeneity of your sample.
Yes, the Nano and Pico detectors in the NT.115 series and NT.Automated instruments are very well suited to measure in complex biological liquids like crude cell extract and serum. You don't have to relinquish on sensitivity or precision when analyzing these types of samples.
Yes, the MST instrument enables you to measure the binding of small molecules with the equally high sensitivity that is observed for protein-protein, protein-DNA interactions or protein-vesicle interactions. This is due to the sensitivity to changes in hydration shell and charge beside the size-dependency typically found in bioanalytical approaches.
Yes, by analyzing the signal amplitude you can distinguish whether two antibodies bind the same or two different epitopes. In addition you can perform competition experiments to map the binding sites.
Yes, working with 70S ribosomes or nucleosomes as well as liposomes with a size of more than 100nm is possible.
Yes, in contrast to other surface-based methods, Surface Acousic Wave is sensitive towards conformational changes and can therefore be applied to quantify binding-induced conformational changes.
